The Impact of Various Exercise Intensities on Energy Expenditure Metabolism

Condition(s) studied

Investigational drug(s) / intervention(s)

Observation without intervention: Observation without intervention

Study summary

The goal of this observational study is to learn about the effects of physical activity in adult on metabolic response. The main question it aims to answer is:

* if physical activity is unlimited to increase the total energy expenditure (TEE)?

Participants will engage in the following measurements:

* Record their daily physical activity by GT3X.
* Measure TEE by doubly-labeled water (DLW) method
* Measure basal energy expenditure(BEE) and thermic effect of food(TEF) by respiratory chamber

Eligibility

Primary outcome measure(s)

• Differences in total energy expenditure (TEE) measured by the doubly labeled water (DLW) method across experimental groups — 14days
  \- The change in TEE will be evaluated using the DLW. Urine samples from all participants in the DLW subset will be stored at -20℃ and shipped on dry ice for analysis in the laboratory of Dr. John Speakman at the University of Aberdeen, or the Shenzhen Institutes of Advanced Technology, Chinese academy of sciences. Isotopes will be measured in benchtop near-infrared isotope gas analyzer, and mean CO2 production will be calculated from isotope ratios using the recently derived equation (Speakman et al 2021: Cell reports medicine). TEE will then be calculated using mean CO2 production using the Weir equation.
• Differences in non-exercise physical activity across experimental groups — 14days
  \- Physical activity of the participants will be recorded using GT3X accelerometer worn near the hip for a consecutive period of 14 days.
• Differences in resting energy expenditure (REE) measured by respiratory chamber across experimental groups — 1 day
  \- REE will be evaluated by respiratory chamber.
• Differences in thermic effect of food (TEF) measured by respiratory chamber across experimental groups — 1 day
  \- TEF will be evaluated by respiratory chamber.
• Differences in exercise energy expenditure (EEE) measured by COSMED across experimental groups — 1 hour
  \- EEE will be evaluated using an indirect calorimetry COSMED.
• Differences in food intake across experimental groups — 2 days
  Photographic and food weighing methods to record food intake for two random days throughout the period of DLW measurement

Trial sites (1)

More Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences trials in China

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