Characterization of Circulating and Tumor-infiltrating Immune Cells in Malignant Brain Tumors

Condition(s) studied

Investigational drug(s) / intervention(s)

Circulating leukocytes immunophenotype: Blood samples will be tested with flow cytometry in order to characterize leukocyte subpopulations and to evaluate the circulating immunophenotype

Tumor sampling: Immunohistochemical analysis will be performed on tumor samples in order to characterize immune T cells distribution within the tumor.

Study summary

The goal of this observational study is to characterize the circulating leukocyte profile and the immune T cells distribution within the tumor in patients with malignant brain tumors and to correlate these findings with the oncological outcome.

Participants will be subjected to blood sampling before surgery and for 12 months of follow-up. Additional sampling and analysis will be performed on tumor samples.

Eligibility

Primary outcome measure(s)

• Baseline leucocytes count — Baseline
  Leucocytes count (10\^3/ml), total lymphocytes count (10\^3/ml)
• Baseline leucocytes immunophenotype — Baseline
  Determination of different leucocyte subpopulations (%)
• Baseline tumor-infiltrating lymphocytes count — After surgery (tumor sampling)
  Infiltrating lymphocytes count (10\^3/ml)
• Baseline tumor-infiltrating leucocytes immunophenotype — After surgery (tumor sampling)
  Infiltrating T-cell subpopulations (%)

Trial sites (1)

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