Development of an Innovative Method to Extract Cord Blood Stem Cells With High Efficiency

Condition(s) studied

Investigational drug(s) / intervention(s)

Umbilical cord blood collection: the study purpose is to collect between 70 to 200 ml of blood for each donor from both caesarean and natural births, post-partum. The umbilical cord will be clamped, as directed by the surgeon performing the procedure, in two places: close to the placenta and close to the baby.

Study summary

The present study has the purpose to develop an innovative method for obtaining HSCs with a high yield. HSCs extracted from UCB using the traditional method followed by several steps of centrifugation will be compared to the new method which exploit a new biocompatible liquid, capable to separate the HSCs without causing mechanical stress to cells. The potential of ozone treatment to stimulate cells proliferation will be also assessed. Since ozone has been identified as an adjuvant to sustain cytoprotective pathways increasing cell performance, HSCs obtained from UCB samples will be treated by flowing ozone at low concentrations (between 2 and 20 μg/mL) in a mixture 1:1 with oxygen.

Eligibility

Primary outcome measure(s)

• Efficacy of HSCs extraction — up to 24 months
  Evaluation of the efficacy of HSCs extraction with a new method which exploit a new biocompatible liquid, capable to separate the HSCs without causing mechanical stress to cells.

Trial sites (1)

More Fondazione IRCCS Policlinico San Matteo di Pavia trials in Italy