Transcriptomic Study of Cutaneous Fibroblasts in Scleroderma

Condition(s) studied

Investigational drug(s) / intervention(s)

Skin samples: * Scleroderma group: skin biopsy for scleroderma patients * Control group: recovery of resected tissue from patients undergoing plastic, reconstructive and aesthetic surgery.

Analysis of fibroblasts: Analysis of samples by RNA sequencing

Study summary

Scleroderma is a complex connective tissue disease involving three interconnected pathophysiological axes: vascular hyperactivity and remodelling, immune system dysfunction and over-activation of fibroblasts at the origin of the fibrosis process in the skin and organs. Given that this pathology occurs preferentially in mature subjects, it is possible to suggest a potential inductive role for senescent fibroblasts, which would be responsible for activating and/or maintaining the immune response and systemic inflammation. Our hypothesis is that fibroblasts play a predominant role in the genesis and maintenance of this pathology.

Eligibility

Primary outcome measure(s)

• genes involved in the various cell activation and signalling pathways — through study completion, an average of 12 months
  number and type of genes involved in different cell activation and signalling pathways, including senescence, which will be differentially expressed in fibroblasts from the skin of control participants and those from the skin of subjects with SSc.

Trial sites (1)

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