Physiotherapy and Rehabilitation Practices: It was planned to give the participants a routine stroke rehabilitation program consisting of 45 minutes of active, passive range of motion exercises and strengthening exercises, balance exercises, walking training and 20 minutes of bicycle ergometry, accompanied by a physiotherapist with at least 5 years of experience in stroke rehabilitation. It was planned to give passive bicycle ergometry to patients without voluntary muscle movement and muscle strength, and active bicycle ergometry to participants with voluntary muscle movement and muscle strength, with the bicycle rehabilitation. It was planned to apply the exercise program to the patients for 5 days a week and for a total of 30 sessions. It will be recommended that they continue the medical treatment they are using during the treatment program. Routine evaluations will be made by the physiatrist before the rehabilitation program starts and 2 tubes of venous blood will be taken from the patients by an experienced nurse
Study summary
The aim of this study is to demonstrate the effect of routine exercise program on neuroplasticity through histone acetylation and gene expression changes in acute stroke survivors from an epigenetic perspective and to investigate the correlation of epigenetic effects with its effects on motor function and quality of life.
Eligibility
Sex
ALL
Min age
25 Years
Max age
70 Years
Healthy volunteers
No
Inclusion Criteria:
1. Participants who applied to Afyonkarahisar Health Sciences University Physical Therapy and Rehabilitation Clinic for rehabilitation purposes and had a stroke for the first time
2. Participants with ischemia as the etiology of stroke
3. Participants between the ages of 25-70
4. Drugs that have been used for at least 1 month and up to 6 months after a cerebrovascular accident
Exclusion Criteria:
1. Participants whose stroke etiology is other than ischemia
2. Participants with clinically significant neurological disease other than stroke
3. Participants with systemic and musculoskeletal diseases that will not tolerate neurological rehabilitation and prevent them from participating
Primary outcome measure(s)
Genetic analyses — Baseline (pre-treatment) and 6 weeks (post-treatment) Real-Time Polymerase Chain Reaction (PCR) method will be used to determine the expression of BDNF, CREB1, GAP43, NTRK2 and SYN1 genes from the peripheral venous blood samples of the participants. The region of interest will be amplified in the Real-Time PCR device using primer pairs specific to the relevant genes. Changes in gene expressions in patients before and after treatment will be determined.
Histone Proteins — Baseline (pre-treatment) and 6 weeks (post-treatment) Leukocytes will be isolated from blood samples before and after exercise. Isolation of histone proteins will be performed from the isolated leukocytes using the relevant isolation procedures. Pre- and post-treatment levels will be compared.
Histone Acetylation Analysis — Baseline (pre-treatment) and 6 weeks (post-treatment) H3K27ac levels in the isolated histone proteins will be determined colorimetrically based on the ELISA method. Pre- and post-treatment levels will be compared.
Trial sites (1)
Facility
City
Region
Status
Afyonkarahisar Health Sciences University
Afyonkarahisar
Turkey (Türkiye)
Recruiting
More Afyonkarahisar Health Sciences University trials in Turkey
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
We use cookies to analyse site traffic and improve your experience. With your consent, we may also use cookies for advertising. You can change your choice at any time.
