Erythrocyte Transport of Lactate During Exercise (TELE Project)

Condition(s) studied

Investigational drug(s) / intervention(s)

Intervallic submaximal exercise test: Intervallic submáximal exercise test on a cycle ergometer: 5 min warm-up at 80% of First Lactate Threshold (LT1), and subsequent sets of 1.5 min at 20% above the Second Lactate Threshold (LT2) alternated with recoveries of 5 min at 80% of LT1, ending with a 20 min recovery at 80% of LT1.

Study summary

The goals of this clinical trial are:

1. to study lactate kinetic between plasma and erythrocytes during an intervallic exercise and its subsequent recovery, considering the blood pH, the genotype for the T1470A polymorphism of the SLC16A1 gene (rs1049434), and the amount of MCT1 in erythrocytes membrane;
2. to analyze the levels of MCT1 in the erythrocytes membrane according to training status and genotype for the T1470A polymorphism of SLC16A1 (rs1049434).

For this, the project will have two phases:

* In phase I, trained participants will perform one maximal incremental test and one intervallic submaximal test with a final active recovery.
* Phase II, levels of the MCT1 protein in the erythrocyte membrane will be quantified from trained and sedentary participants.

Eligibility

Primary outcome measure(s)

• Plasma-Erythrocyte lactate difference — Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years
  Difference between lactate concentration inside the erythrocytes and lactate concentration in plasma, expressed in mMol/L (Millimoles Per Liter), from venous blood extracted during the intervallic test from the antecubital vein.
• Level of Monocarboxylate Transporter 1 in erythrocytes membrane — Through study completion, average of 5 years, an average of 2 months per participant
  Protein abundance of the monocarboxylate transporter 1 in erythrocyte membranes. It is measured using flow cytometry, from venous blood extracted from the forearm in rest state.

Trial sites (2)

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