The role of the intestinal microbiota on health is now well recognised, and diet is one of the factors influencing the maintenance of a healthy intestinal microbiota. More specifically, the consumption of fruit, vegetables and cereals is associated with good health and, in particular, with an increase in the relative abundance of bacterial groups described as beneficial. To date, a large number of bacterial species in the intestinal microbiota have yet to be isolated and cultivated, which limits the characterisation of their potential health benefits. In order to isolate and cultivate these intestinal bacteria, it is particularly relevant to recruit individuals with a high-fibre diet.
Eligibility
Sex
ALL
Min age
20 Years
Max age
50 Years
Healthy volunteers
Accepted
Inclusion Criteria:
* Healthy adult subjects ;
* Age between 20 and 50 (limits included) ;
* BMI between 18.5 and 25 kg/m2 (excluding limits) ;
* Consumption of more than 5 portions of fruit and vegetables a day;
* Intake of at least 25g of dietary fibre per day;
* A varied diet with little processed food.
Exclusion Criteria:
* Antibiotics and/or transit modulators taken in the 3 months prior to sampling;
* Known pathologies;
* Current treatment;
* History of digestive surgery having an impact on the microbiota, deemed incompatible with the study (bariatric surgery);
* Consumption of food supplements based on protein preparations, probiotics or prebiotics;
* Smoking (even occasionally);
* Alcohol consumption \> 2 glasses (i.e. 20 g) per day;
* Intense sporting activity;
* Pregnant or breast-feeding women;
* Persons under legal protection (curatorship, guardianship, etc.);
* Volunteers taking part in another intervention study;
* Persons refusing to be registered on the French Ministry of Health's Fichier National des Volontaires Sains (National Register of Healthy Volunteers);
* Subject not affiliated to the social security system;
* Subject in a period of exclusion from a previous study or having received a total amount of compensation exceeding 6000 euros over the 12 consecutive months prior to the start of the trial (after verification in the Fichier des Volontaires pour la Recherche Biomédicale).
Primary outcome measure(s)
Isolation and culture of one isolate from each target species — Up to 3 years Successful isolation and culture of at least one isolate from each target species previously identified through metagenomic data analyses.
Trial sites (1)
Facility
City
Region
Status
CRNH Auvergne
Clermont-Ferrand
France
Recruiting
More Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement trials in France
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
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