Protein Quality of Chlorella and Fababean Meat Analogue
Investigational drug(s) / intervention(s)
Single meal containing 20 g of 15N intrinsically labelled protein from honey chlorellaSingle meal containing 20 g of 15N intrinsically labelled protein from faba bean meat analogue
Single meal containing 20 g of 15N intrinsically labelled protein from honey chlorella: Ingestion of a 15N labeled test meal. Subjects are equipped with a nasoileal tube. 15N is followed in biological fluids (plasma, effluents, urines).
Single meal containing 20 g of 15N intrinsically labelled protein from faba bean meat analogue: Ingestion of a 15N labeled test meal. Subjects are equipped with a nasoileal tube. 15N is followed in biological fluids (plasma, effluents, urines).
Study summary
The goal of the project is to determine the protein quality of alternative protein sources, honey chlorella and Faba bean, ingested as mixed meals in healthy subjects equipped with naso-ileal tube. For this purpose, Chlorella and Faba bean are intrinsically labelled with 15N. Faba bean is processed to produce a meat analogue. Chlorella is introduced in a drink. The protein quality is determined by following the digestive and metabolic fate of 15N during a 8h postprandial investigation.
Eligibility
Sex
ALL
Min age
18 Years
Max age
60 Years
Healthy volunteers
Accepted
Inclusion Criteria:
* 18\<BMI\<30
* Good health (WHO=0)
* For woman: use of birth control
Exclusion Criteria:
* food allergy
* allergy against latex
* pregnant women
* Positive serology for AgHBS, AcHbc, HCV, HIV
* Anemia
* Excessive alcohool consumption
* Hypertension, diabetes, digestive, hepatic or renal diseases, cardiac disease
* Exercice\>7h/week
* Blood donation within 3 months before the study
* Participation to another clinical study within 3 months before the study
Primary outcome measure(s)
Ileal digestibility of protein and amino acids — -30 minutes to 8 hours after the test meal The measurement of nitrogen (mmol) and 15N enrichment (atom %) in ileal samples allow to determine the amount of dietary nitrogen in the lumen. Dietary nitrogen that is recovered in the ileal samples are then considered as non absorbed.
Net Postprandial Protein Utilization (NPPU) — 0 to 8 hours after the test meal The measurement of urea (mmol) and 15N enrichment of urea (atom %) allow to determine the amount of dietary N that was transferred to urea, thus accounting for metabolic losses. Metabolic losses of dietary N, together with digestive losses of dietary N, are not retained in the body.
Ileal digestibility of amino acids — -30 minutes to 8 hours after the test meal The measurement of amino acid concentration (mmol) and their individual 15N enrichment (atom %) allow the determination of dietary amino acids remaining in the lumen. Dietary amino acids that are recovered in the ileal samples are then considered as non absorbed.
Trial sites (1)
Facility
City
Region
Status
Research Center on Volunteers
Bobigny
France
Recruiting
More Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement trials in France
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
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