Initial periodontal treatment: DOS samples will be taken before and after treatment
Study summary
The goal of this observational study is to investigate periodontal inflammatory mediators in systemically healthy children aged 8-13 years with and without molar-incisor hypomineralization (MIH) who require initial periodontal treatment due to gingivitis.
The main questions it aims to answer are:
Are the levels of periodontal inflammatory mediators in gingival crevicular fluid different between children with MIH and those without MIH? Is the presence and severity of MIH associated with clinical periodontal parameters and dentin hypersensitivity?
Researchers will compare children with MIH to children without MIH to determine whether MIH is associated with changes in periodontal inflammatory mediator levels and periodontal clinical findings.
Eligibility
Sex
ALL
Min age
8 Years
Max age
13 Years
Healthy volunteers
Accepted
Inclusion Criteria:
* Systemically healthy children aged 8-13 years (both sexes).
* Children who demonstrate adequate cooperation for dental treatment and study procedures (Frankl Behavior Rating Scale score ≥3).
* Study group: Children with molar-incisor hypomineralization (MIH) affecting at least three permanent first molars and requiring initial periodontal treatment for gingivitis.
* Control group: Children without molar-incisor hypomineralization who require initial periodontal treatment for gingivitis.
* Presence of gingivitis requiring initial periodontal therapy.
Exclusion Criteria:
* Use of antibiotics within the previous 1 month.
* Radiographic evidence of periodontal destruction.
* History of periodontal treatment within the previous 3 months.
* Presence of systemic, chronic, or metabolic diseases.
* Ongoing active orthodontic treatment.
* Developmental enamel defects other than molar-incisor hypomineralization.
* History of premature birth.
Primary outcome measure(s)
Concentration of proinflammatory and anti-inflammatory cytokines in gingival crevicular fluid — Baseline, 1 month after initial periodontal therapy, and 3 months after initial periodontal therapy Quantitative concentrations (pg/mL) of IL-1α, IL-1β, IL-6, IL-8, TNF-α, IFN-γ and IL-4, IL-10, IL-13, TGF-β1 measured in gingival crevicular fluid using enzyme-linked immunosorbent assay (ELISA).
Concentration of matrix metalloproteinases in gingival crevicular fluid — Baseline, 1 month after initial periodontal therapy, and 3 months after initial periodontal therapy Quantitative concentrations (pg/mL) of MMP-2, MMP-3, MMP-8, MMP-9, MMP-20, and TIMP-1 measured in gingival crevicular fluid using ELISA. Measurements will be obtained at baseline (before initial periodontal therapy) and after completion of initial periodontal therapy.
Total oxidant status and total antioxidant status in gingival crevicular fluid — Baseline, 1 month after initial periodontal therapy, and 3 months after initial periodontal therapy Total Oxidant Status (TOS) and Total Antioxidant Status (TAS) measured by spectrophotometric analysis at baseline (before initial periodontal therapy) and after completion of initial periodontal therapy.
Trial sites (1)
Facility
City
Region
Status
Marmara Üniversitesi Recep Tayyip Erdoğan Külliyesi Sağlık Yerleşkesi, Diş Hekimliği Fakültesi
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
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