Atherosclerosis Cardiovascular DiseaseObesityMetabolic SyndromeMetabolic DisordersInflammationNeurodegenerationNeurodegenerative DiseaseAlzheimer s Disease
Investigational drug(s) / intervention(s)
White WineRed WineWater
White Wine: Chardonnay, 4 mL/kg body weight
Red Wine: Cabernet Sauvignon, 4 mL/kg body weight
Water: Still water, 4 mL/kg body weight
Study summary
This study aims to investigate how moderate wine consumption influences circulating extracellular vesicles (EVs) in healthy adults. EVs are small particles released by cells that carry proteins, lipids, and genetic material, and play important roles in communication between cells. Participants will consume a single serving of red or white wine, and blood samples will be collected before and after consumption to study changes in the composition and function of EVs. The study will also assess how these EVs affect vascular, immune, and brain-related cells. The results are expected to improve our understanding of how moderate wine intake contributes to cardiovascular and brain health.
Eligibility
Sex
ALL
Min age
35 Years
Max age
36 Years
Healthy volunteers
Accepted
Inclusion Criteria
* Healthy adult men and women aged 35 to 65 years.
* Body Mass Index (BMI) between 18.5 and 29.9 kg/m².
* Non-smokers or ex-smokers for at least 12 months.
* Moderate alcohol consumers, defined as ≤2 units/day for men and ≤1 unit/day for women.
* Normal fasting glucose and lipid profile at screening.
* Willing and able to refrain from alcohol, polyphenol-rich foods, and intense exercise for 48 hours before each study visit.
* Able to understand the study procedures and provide written informed consent.
Exclusion Criteria
* History or clinical evidence of cardiovascular, hepatic, renal, thyroid, gastrointestinal, or metabolic diseases (including diabetes, dyslipidemia, or hypertension).
* Use of medications or supplements known to affect glucose, lipid, or inflammatory metabolism (e.g., statins, corticosteroids, anti-inflammatory drugs).
* Pregnancy or breastfeeding.
* Recent blood donation (within the last 3 months) or planned blood donation during the study period.
* Major weight change (\>5% of body weight) within the last 3 months.
* Participation in another clinical or biomedical study within the previous 3 months.
* Known allergy or intolerance to wine, alcohol, or its components (e.g., sulfites).
* History of alcohol abuse or inability to abstain from alcohol outside the study context.
* Reluctance to receive information about incidental health findings arising from the study.
* Any condition judged by the investigators to limit compliance or increase study risk (e.g., psychiatric disorders, inability to adhere to fasting requirements).
Primary outcome measure(s)
Concentration of circulating extracellular vesicles — Baseline (0 hours), postprandial peak (2 hours after beverage intake), and post-peak (6 hours after beverage intake). Concentration of plasma-derived extracellular vesicles measured after acute intake of red wine, white wine, or water. Extracellular vesicles will be isolated by immunoaffinity methods and quantified using nanoparticle-based analysis. Results will be expressed as particles per milliliter (particles/mL).
Mean size of circulating extracellular vesicles — Baseline (0 hours), postprandial peak (2 hours after beverage intake), and post-peak (6 hours after beverage intake). Mean diameter of plasma-derived extracellular vesicles measured after acute intake of red wine, white wine, or water. Extracellular vesicle size will be assessed using dynamic light scattering. Results will be expressed in nanometers (nm).
Trial sites (1)
Facility
City
Region
Status
Av. de Sánchez Pizjuán, s/n, 41009 Sevilla Facultad de Medicina . Universidad de Sevilla
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
We use cookies to analyse site traffic and improve your experience. With your consent, we may also use cookies for advertising. You can change your choice at any time.