The assessment of microcirculatory function plays a pivotal role in perioperative medicine and intensive care. One promising method for evaluating microcirculatory function is the microscopic analysis of the oral mucosal microvasculature. However, this method is not currently fully validated.
The aim of this study is to assess the repeatability of sublingual microcirculation measurements using the CytoCam-IDF device (Braedius Medical).
Eligibility
Sex
ALL
Min age
18 Years
Max age
—
Healthy volunteers
Accepted
Group 1: Healthy Volunteers
Inclusion Criteria:
* ASA Physical Status I-II
* Age 18-40 years
Exclusion Criteria:
* Chronic diseases
* Chronic medication use
* Pregnancy
* Use of stimulants (caffeine, nicotine, alcohol) within 12 hours prior to the study
* Lack of consent to participate
Group 2: Patients Hospitalized in the ICU
Inclusion Criteria:
* Age \> 18 years
* Hospitalization in the Intensive Care Unit (ICU)
* Requirement for vasoactive drugs (minimum norepinephrine equivalent dose \>0.1μg/kg / min )
* Mechanical ventilation via an endotracheal tube
Exclusion Criteria:
* Head and craniofacial trauma
* Pregnancy
* Intensive fluid therapy (\>200ml/h)
* Changes in vasoactive drug dosages within 30 minutes prior to study inclusion
* Hypothermia (body temperature \<35.5 oC)
* Fever (body temperature \>38.5oC)
Primary outcome measure(s)
FCD (Functional Capillary Density) — From enrollment every 5 minutes (3 measurements per operator) - approx. 20 min. Sublingual Microcirculation Assessment Sublingual microcirculation will be assessed using the CytoCam-IDF camera (Braedius Medical, Huizen, The Netherlands). The measurement technique involves gently applying the microscope probe with a disposable cap to the sublingual mucosa and recording short video sequences. The device utilizes green LED light to visualize moving blood cells, allowing for subsequent computerized analysis of capillary density and flow quality within the microvessels. Two operators will perform 3 series of measurements at 5-minute intervals with Cytocam - IDF microcirculation camera. Each series will consist of measurements taken from 3 adjacent sites. All measurements will be performed by the same two individuals
Unit of measurement: mm/mm\^2
MFI (Microvascular Flow Index) — From enrollment every 5 minutes (3 measurements per operator) - approx. 20 min. Sublingual Microcirculation Assessment Sublingual microcirculation will be assessed using the CytoCam-IDF camera (Braedius Medical, Huizen, The Netherlands). The measurement technique involves gently applying the microscope probe with a disposable cap to the sublingual mucosa and recording short video sequences. The device utilizes green LED light to visualize moving blood cells, allowing for subsequent computerized analysis of capillary density and flow quality within the microvessels. Two operators will perform 3 series of measurements at 5-minute intervals with Cytocam - IDF microcirculation camera. Each series will consist of measurements taken from 3 adjacent sites. All measurements will be performed by the same two individuals
Unit of measurement: 0 - no flow / 1 -intermittent flow / 2 - sluggish flow / 3 - continuous flow
HI (Heterogeneity Index) — From enrollment every 5 minutes (3 measurements per operator) - approx. 20 min. Sublingual Microcirculation Assessment Sublingual microcirculation will be assessed using the CytoCam-IDF camera (Braedius Medical, Huizen, The Netherlands). The measurement technique involves gently applying the microscope probe with a disposable cap to the sublingual mucosa and recording short video sequences. The device utilizes green LED light to visualize moving blood cells, allowing for subsequent computerized analysis of capillary density and flow quality within the microvessels. Two operators will perform 3 series of measurements at 5-minute intervals with Cytocam - IDF microcirculation camera. Each series will consist of measurements taken from 3 adjacent sites. All measurements will be performed by the same two individuals
Unit of measurements none, relative measurement
Trial sites (1)
Facility
City
Region
Status
Medical University of Gdanks
Gdansk
Poland
More Medical University of Gdansk trials in Poland
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
We use cookies to analyse site traffic and improve your experience. With your consent, we may also use cookies for advertising. You can change your choice at any time.