High intensity interval trainingmoderate intensity continuous trainingMediterranean diet
High intensity interval training: Patients will engage in high-intensity interval training using heart rate reserve (HRR) to determine exercise intensity. The program includes three sessions per week for 12 weeks, featuring three 3-minute high-intensity intervals at 70-90% HRR, with 2 minutes of active recovery in between. Each 30-minute session will be treadmill-based under supervision, incorporating warm-up and cool-down periods. Participants will also follow a Mediterranean diet.
moderate intensity continuous training: The program will be in form of:
* Frequency: 3 sessions/week for 12 weeks.
* Intensity: Moderate intensity continues training at 40- 60% HRR.
* Duration: the session will last 30 minutes per session. Patients will be instructed to follow Mediterranean Diet.
Mediterranean diet: the composition of the Mediterranean diet, emphasizing that vegetables and fruits make up the largest portion at 35%, followed by whole grains (20%) and fish and seafood (15%). Legumes and nuts account for 10%, while olive oil and dairy products (cheese and yogurt) each contribute 7%. Smaller portions include poultry and eggs (4%) and red meat and sweets (2%), reflecting the diet's focus on plant-based foods, healthy fats, whole grains, and moderate consumption of animal products, with minimal intake of red meat and sugary foods.
Study summary
To compare High-Intensity Interval Training versus moderate intensity continuous training on gut microbiota in Non- Alcoholic Fatty Liver.
Eligibility
Sex
ALL
Min age
40 Years
Max age
60 Years
Healthy volunteers
No
Inclusion Criteria:
1. Patients from both genders aged 40 - 60 years old diagnosed with Non-alcoholic Fatty Liver disease by ultrasonography.
2. Body mass index will range from 30 to 34.9 kg/m².
3. Patients with central obesity (waist circumference 90 cm for men and 85 cm for women).
4. Patients who have taken no regular exercise in the last year (\<3 times/week and 30 min/time.
5. No antibiotic or probiotic use for at least last 3 months.
6. Medical drugs such as metformin, sulfonylureas, statins, and blood pressure medication were eligible, as well as stable.
Exclusion Criteria:
1. History of liver cirrhosis or other chronic liver diseases.
2. Use of medications affecting gut microbiota (e.g., laxatives, metformin, proton pump inhibitors).
3. Recent gastrointestinal infections or surgeries.
4. Diabetes Mellitus type 1 or uncontrolled type 2.
5. Cardiovascular, respiratory, or musculoskeletal disorders limiting exercise capacity.
6. Active smokers.
7. Pregnancy or breastfeeding.
8. Uncontrolled hypertension.
9. Communication disorders.
10. Bleeding disorders.
Primary outcome measure(s)
assessment of short chain free fatty acids butyrate and acetate — at baseline and after 12 weeks For short chain free fatty acids analyses, fecal sample will be separated. Applying four analytes, including total Short Chain Fatty Acids, acetate, propionate, and butyrate, will be targeted. Final concentrations will be calculated based on internal standards and are expressed as micromoles per gram of wet feces (μmol/g). Techniques and predictive approaches will be used across. gas chromatography will be used to quantify Short Chain Fatty Acids from fecal samples
assessment of lipid profile — at baseline and after 12 weeks Fasting blood samples were collected after an overnight fast, and serum lipid levels were analyzed using standard enzymatic methods with an automated biochemical analyzer. The lipid profile included triglycerides, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol to assess lipid metabolism and cardiovascular risk.
Trial sites (1)
Facility
City
Region
Status
out-patient clinic, faculty of physical therapy, Horus university
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
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