blood sampling: Whole blood samples (5 mL per each time point, a total of 20 mL per subject) will be collected
Study summary
Itch is defined as an unpleasant sensation that evokes the desire to scratch. Chronic itch, defined as itch persisting for more that 6 weeks, is a key symptom in many diseases severely affects the quality of life of the affected patients and represents a substantial economic burden for the society. At present, there are no effective treatments for chronic itch diseases. Moreover, chronic itch patients express differences in their genes compared with people who do not suffer from itch.
With this experiment, we wish to analyse differences in genes expression in healthy volunteers who have been exposed to artificial itch from small plant needles from the plant cowhage (mucuna pruriens) and compare them to controls who have not been exposed to itch.
Eligibility
Sex
ALL
Min age
18 Years
Max age
60 Years
Healthy volunteers
Accepted
Inclusion Criteria:
* Healthy men and women
* 18-60 years
* Speak and understand English
Exclusion Criteria:
* Pregnancy or lactation
* Drug addiction defined as any use of cannabis, opioids, or other addictive drugs
* Previous or current history of neurological (e.g., neuropathy), immunological (e.g., asthma, immune deficiencies, arthritis) musculoskeletal (e.g., muscular pain in the upper extremities), cardiac disorder, or mental illnesses that may affect the results,
* Lack of ability to cooperate
* Current use of medications that may affect the trial such as antihistamines, antipsychotics, and pain killers, as well as systemic or topical steroids
* Allergy to lidocaine
* Skin diseases (e.g., atopic dermatitis, pruritus nodularis, eczema, psoriasis)
* Moles, scars, or tattoos in the area to be treated or tested.
* Consumption of alcohol or painkillers 24 hours before the study days and between these
* Smoking
* Acute or chronic pain
* Participation in other trials within one week of study entry (four weeks in the case of pharmaceutical trials)
Primary outcome measure(s)
miRNA expression over time — Before active/inactive cowhage Plasma miRNA evaluation
miRNA expression over time — 30 minutes post-Active/inactive cowhage Plasma miRNA evaluation
miRNA expression over time — 3 hours post-active/inactive cowhage Plasma miRNA evaluation
miRNA expression over time — 24 hours post-active/inactive cowhage Plasma miRNA evaluation
Protein expression over time — Before active/inactive cowhage Plasma protein evaluation
Protein expression over time — 30 minutes post-active/inactive cowhage Plasma protein evaluation
Protein expression over time — 3 hours post-active/inactive cowhage Plasma protein evaluation
Protein expression over time — 24 hours post-active/inactive cowhage Plasma protein evaluation
This page summarises publicly available registry data for informational purposes — not medical advice. Eligibility is determined by each study team; patients should discuss participation with their clinician.
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