Rapid Detection of Pseudomonas Aeruginosa in Bronchoalveolar Lavage Fluid Using Label-free Single-particle Imaging Technology and Assessment of Post-treatment Efficacy in Patients With Pseudomonas Aeruginosa Infection

Condition(s) studied

Investigational drug(s) / intervention(s)

bronchoscopy: We will perform bronchoscopy and collect bronchoalveolar lavage fluid (BALF)

Study summary

The aim of this study is to observe specimens of bronchoalveolar lavage fluid from patients using a reflection enhanced dark-field scattering microscopy. By observing parameters such as the size, morphology, scattering intensity, and movement speed of pathogens, combined with the clinical characteristics of patients, a rapid diagnosis of Pseudomonas aeruginosa infection can be made. At the same time, the bronchoalveolar lavage fluid samples of patients infected with Pseudomonas aeruginosa before and after treatment were compared, and the quantity and vitality of Pseudomonas aeruginosa were observed to judge the efficacy of antibiotics.

Eligibility

Primary outcome measure(s)

• The size of pathogens — From enrollment to the end of treatment at 2 weeks
  Measure the size of pathogens
• scattering intensity of pathogen — From enrollment to the end of treatment at 2 weeks
  Determine the shape of pathogens through scattering intensity
• movement speed of pathogens — From enrollment to the end of treatment at 2 weeks
  Measure the movement speed of pathogens, such as flagellates like Pseudomonas aeruginosa, which have a fast movement speed

Trial sites (1)

More The First Affiliated Hospital with Nanjing Medical University trials in China

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