MRD in High-risk EBC

Inclusion Criteria: * Informed consent signed * Age ≥18 years old * Undergoing breast surgery, the surgical specimen was diagnosed as invasive breast cancer by the central laboratory and met any of the following conditions: 1. Hormone receptor-positive/HER2-negative breast cancer, the number of lymph node metastases ≥ 4; 2. HER2-positive breast cancer, lymph node metastasis≥4; 3. Triple negative breast cancer, the number of lymph node metastases ≥ 1; * ECOG 0-1 * Willing to cooperate in clinical research-related treatment and follow-up, with good patient compliance Exclusion Criteria: * Confirmed distant metastases site * History of other malignancy * Psychopaths or other reasons unable to comply with treatment * Concomitant uncontrolled lung disease, severe liver and kidney disease, severe infection, active peptic ulcer requiring treatment, coagulation disorders, connective tissue disease, or bone marrow suppression, and cannot tolerate chemotherapy and other related treatments * HIV carrier or HBC/HCV infected * Current or recent (within 30 days prior to enrollment) use of participation in another investigational program or use of another investigational drug