Application of Nanopore Adaptive Sequencing

Condition(s) studied

Investigational drug(s) / intervention(s)

NAS, NGS and Laboratory tests: Using three diffrent detection methods and comparing their efficiency

Study summary

Infection post liver transplantation is an important factor in the death in patients. The traditional method of diagnosing infection post liver transplantation is laboratory tests. But the sensitivity and specificity of blood tests is poor. Next-generation sequencing (NGS) has greater detection rate for mycobacterium tuberculosis, anaerobes and fungi and greater sensitivity compare with blood tests. However use of NGS is limited because of the short read-length. Oxford nanopore adaptive sequencing (NAS) method is the Third Revolution in Sequencing Technology. For each 1 Gbp of data, NAS sequencing detected 45 times more microbiome sequences than Nanopore standard sequencing and 2.5 times more than Illumina sequencing. The purpose of this study is to compare NAS with NGS and laboratory tests for the diagnostic rate of infection post liver transplantation.

Eligibility

Primary outcome measure(s)

• Diagnosed infection post liver transplantation — Post liver transplantation before patients discharge from hospital, an average of 1 months.
  Any pathogens are detected in alveolar lavage fluid, peritoneal drainage or blood before patients discharge from hospital, an average of 1 months

Trial sites (1)

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