Establishment of a Cohort of Patients With Sepsis-associated Encephalopathy (SAE)

Inclusion criteria: 1. sepsis 3.0 diagnostic criteria; 2. Estimated length of hospital stay\> 24h; 3. ages 18-89; 4. Acute brain dysfunction: delirium, coma, epilepsy, focal neurological deficit; 5. Patients who meet the non-infectious SIRS diagnostic criteria Exclusion criteria: 1. Diagnosis of patients with brain injury before admission, including Alzheimer's disease, craniocerebral injury, etc .; 2. Primary brain injury (cerebral hemorrhage, cerebral infarction, etc.), secondary brain injury (liver brain, lung brain, uremia encephalopathy, pancreatic encephalopathy, metabolic encephalopathy, Wake encephalopathy, etc.); 3. pregnant and lactating women; 4. Those who have undergone bypass surgery in the past 3 months; 5. Hearing and vision impairment; 6. mental illness and melanoma; 7. Abnormal coagulation, active bleeding 8. Patients with infection at lumbar puncture site 9. Meningeal leukemia patients 10. Patients with other types of encephalopathy